High-throughput sequencing

Keywords: DNA Sequencing, high throughput, DNA libraries, bioinformatics

Next Generation Sequencing instruments yield several gigabases of short fragment (35-50 nucleotides) sequences, within a few days.

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This novel technology has multiple applications:

    * Large scale resequencing of whole genomes (Re-SEQ) allowing to identify single nucleotide polymorphism (SNP), indels, large duplications, deletions, insertions, inversions, translocations, changes in ploidy, etc…)
    * Targeted sequencing of a large number of samples (Target-SEQ)
    * mapping of methylation sites (M-SEQ)
    * Mapping of protein binding sites and epigenetics modifications of chromatin (ChIP-SEQ)
    * Identification of small RNA (sRNA-SEQ)
    * Monitoring of gene expression (RNA-SEQ)
    * Identification of distant chromosomal interactions (Chromosome Conformation Capture)

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The high throughput sequencing platform (SEQ-HD) is located in the Institut Curie, 26 rue d'Ulm, Hospital Building B, room 104.

The platform is equipped with a SOLiDTM instrument (version 2) from Applied Biosystems and all complementary equipments to prepare the DNA libraries. Primary analyses of the sequences are performed by the Institut Curie Bioinformatics platform (U900 INSERM - Mines ParisTech - Institut Curie).

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Access and project workflow

The platform is dedicated to the laboratories of the Institut Curie and of the Cancéropôle Ile-de-France. It is also accessible to the other teams of the Montagne-Sainte-Geneviève and the Région Ile-de-France (contact us).

The instrument and the additional equipments of the platform are not on free access. The Bioinformatics platform of the Institut Curie performs the first level analyses (reports quality control of the run and mapping of the sequences on the reference genome) and provides tools for second level analyses and, whenever needed, dedicated assistance upon request (contact: bioinfo-ngs-contact@curie.fr). Costs will be invoiced by the Institut Curie.

The platform will handle all the above SOLiDTM system applications.

General information on the instrument specifications, the diversity of applications, the experimental methods and the bibliographical references are available at Applied Biosystems.