Séminaire Physico-Chimie
13 avril
2022

Chromatin as a biomolecular condensate

Le 13 avril - 11h-12h
Centre de recherche - Paris - Amphithéâtre Marie Curie
Pavillon Curie, 11 rue Pierre & Marie Curie, Paris 5ème
Description

Cells package their genomic DNA into a complex with proteins called chromatin. Chromatin is partitioned into subcompartments that differ from each other with respect to their compaction, molecular composition and biological function. One example are the micron-sized spherical heterochromatin foci that are present in several differentiated cell types and that are readily visible under the microscope. It is a key question how such compartments can form even though diffusion constantly mixes the nuclear interior. One biophysical mechanism that can explain this phenomenon is liquid-liquid phase separation, which drives the formation of liquid droplets around nucleation sites on chromatin. Another one is bridging-induced polymer-polymer phase separation, which condenses chromosomal segments into compact globules. I will briefly discuss these mechanisms and will present recent microscopy-based experiments and computational analyses that assess the properties of condensates in the test tube and of heterochromatin foci in living cells. I will compare these experiments to the different models and touch upon the implications for the dynamic regulation of chromatin subcompartments.

At Amphi Curie and on TEAMS

Orateurs
Fabian
Erdel
CBI, Toulouse
Invité(es) par
Antoine
Coulon