Investigating cellular logistics with live-cell STED super-resolution microscopy
In the lab we use gene editing and live-cell super-resolution microscopy to study the mechanisms of membrane homeostasis in health and disease. To be able to image physiological molecular processes in the crowded cellular cytoplasm in living cells, we developed labelling methods for stimulated emission depletion (STED) super-resolution imaging in living cells and a pipeline for the rapid generation of CRISPR-Cas9 knock ins. These methods unlocked the possibility to image dynamics at sub-50 nm spatial resolution and under near-native cellular conditions. Dynamic nanoscale microscopy of endogenously tagged machinery is revealing novel cellular roles for ARF GTPases, one of the major family of regulators of cellular membrane homeostasis. Defining the role of ARFs led to the discovery of unexplored sorting mechanisms between the endoplasmic reticulum and the Golgi apparatus. Different ARFs mediate bi-directional transport of anterograde and retrograde cargoes via a dynamic network of connecting nano-tunnels and different types of ER-Golgi-intermediate compartments. In a similar way, tubular-vesicular trans-Golgi-network-derived elements interact with downstream endosomal compartments to coordinate secretory trafficking.
THE CONFERENCE WILL STAND IN AMPHI AND SIMULTANEOUSLY BY ZOOM:
Here is the link: https://cnrs.zoom.us/j/95051592795?pwd=LytnaVl6R0dsWXV3dGxtWVlaRUk3Zz09