Tirer des Nanotubes membranaires de GUVs

Coline Prévost*^1,2,3, Feng-Ching Tsai*^1,4, Patricia Bassereau^1,4, Mijo Simunovic^1,5

¹Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168, ²Department of Genetics and Complex Diseases, T. H. Chan School of Public Health, Harvard Medical School, ³Department of Cell Biology, Harvard Medical School, ⁴Sorbonne Universités, UPMC University Paris 06, ⁵Center for Studies in Physics and Biology, The Rockefeller University

* These authors contributed equally

Please, click on the following link to see the video of this work.

Many proteins in the cell sense and induce membrane curvature. We describe a method to pull membrane nanotubes from lipid vesicles to study the interaction of proteins or any curvature-active molecule with curved membranes in vitro.