Spliceosome Mutations in Myeloid Malignancy

The primary goal of Dan Larson's laboratory is to understand gene expression in eukaryotic cells, starting from the mechanistic behavior of individual macromolecules and proceeding to their regulation in cells and tissue.
The laboratory utilizes a battery of biophysical, molecular and genomic approaches, including single-molecule microscopy, RNA visualization in fixed and living cells, computational modeling of gene regulation, and nascent RNA sequencing. Dr. Larson helped pioneer in vivo single-molecule studies of transcription and splicing.

The view that has emerged from these studies is that gene regulation is a dynamic process resulting in stochastic variation within populations. Current work is focused on applying these experimental and theoretical approaches to the study of hematopoiesis in health and disease through the trans-NIH Myeloid Malignancies Program.