Decoding transcriptional regulation

10 octobre - 10h00 - 23h59

Centre de recherche - Paris

Amphithéâtre Hélène Martel-Massignac (BDD)

11 rue Pierre et Marie Curie - 75005 Paris

Description

In higher eukaryotes, genes are expressed dynamically in complex spatial and temporal patterns, which are progressively refined to set up body plans and define specific cell-types. The information about when and where each gene is to be expressed is encoded in the sequences of promoter-, enhancer- and silencer regions and realized by transcription factor and cofactor proteins.

I am presenting our work towards understanding the how this regulatory information is sequence-encoded and how cells utilize this information with transcription factor and cofactor proteins. We characterize regulatory sequences by functional screens in Drosophila S2 cells and by a genome-scale candidate testing approach in developing Drosophila embryos. We then use deep-learning approaches to model the sequence-to-function relationship for enhancers and build synthetic enhancers de novo. We also employ motif analysis and mutagenesis, biochemistry and mass spectrometry as well as transcriptome-measurements to dissect functions and mechanisms of regulatory elements, including silencers.

Orateurs

Alexander STARK

Research Institute of Molecular Pathology (IMP)

Invité(es) par

Raphaël MARGUERON

Institut Curie

Allison BARDIN

Institut Curie

Une question sur le séminaire ?

Raphaël MARGUERON

Raphael.Margueron@curie.fr