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- Single residues in the histone core domain guide transcription
Single residues in the histone core domain guide transcription
Centre de recherche - Paris
Salle P. FAVARD de l'ENS
ENS, 46 rue d'Ulm, Paris 5ème
Description
Nucleosomes, as core unit of chromatin, not only package DNA but have long been thought to suppress transcription by acting as a structural barrier. However, once begun, transcription can readily proceed in the presence of nucleosomes, suggesting this model is insufficient. Here, we establish that the ultra-conserved core domain of the ancestral histone H2A.Z informs transcription elongation via direct interaction of its loop 2 region with the RNA polymerase II complex. Interrogating histone H2A sequences representing more than a billion years of eukaryotic evolution in a single synthetic host, we identify the subunit of the complex that can distinguish even single-residue substitutions defining variants of H2A, and control transcription elongation speed and processivity. Our results place the histone core domain at the origin of eukaryotic gene expression, establishing it as a powerful force shaping transcription.
Organisateurs
Caroline Audouin
Institut Curie
Orateurs
Frédéric Berger
Gregor Mendel Institute of Molecular Plant Biology Austrian Academy of Sciences Vienna, Austria
Invité(es) par
Ines Drinnenberg
Institut Curie