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- 2C-Cas9: a versatile tool for clonal analysis of gene function
2C-Cas9: a versatile tool for clonal analysis of gene function
Auteurs
Vincenzo Di Donato, Flavia De Santis, Thomas O. Auer, Noé Testa, Héctor Sánchez-Iranzo, Nadia Mercader, Jean-Paul Concordet, Filippo Del Bene
Résumé
CRISPR/Cas9-mediated targeted mutagenesis allows efficient generation of loss-of-function alleles in zebrafish. To date, this technology has been primarily used to generate genetic knockout animals. Nevertheless, the study of the function of certain loci might require tight spatiotemporal control of gene inactivation. Here, we show that tissue-specific gene disruption can be achieved by driving