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  • Abstract 1442: Prognostic value of Dicer expression in human breast cancers and association with the mesenchymal phenotype

Abstract 1442: Prognostic value of Dicer expression in human breast cancers and association with the mesenchymal phenotype

15 avr. 2010Clinical Research

DOI : 10.1158/1538-7445.am10-1442

Auteurs

Caroline Moyret-Lalle, Gael Grelier, Christine Lasset, Nicolas Voirin, Sylvie Chabaud, Isabelle Treilleux, Alain Puisieux

Résumé

Abstract

Introduction:

Dicer, a ribonuclease, is the key enzyme required for biogenesis of miRNAs and small interfering RNAs (siRNAs). It was previously shown that Dicer is essential for mammalian development and cell differentiation. Recent evidence indicates that Dicer may be involved in tumorigenesis. However, no studies have examined the clinical significance of Dicer at both RNA and protein levels in breast cancer.

Methods:

In this study, the biological and prognostic value of Dicer expression was assessed in breast cancer cell lines, breast cancer progression cellular-models, and in two independent and well-characterised set of breast carcinomas with long term follow-up using tissue microarray and quantitative reverse transcription-PCR.

Results:

We have found that Dicer protein expression is significantly associated with hormone receptors status in breast tumours. Dicer mRNA expression appeared to have an independent prognostic impact in metastatic disease. In the breast cancer cell lines, lower Dicer expression was found in cells harbouring a mesenchymal phenotype and in metastatic bone derivatives of a breast cancer cell line. These findings suggested that down-regulation of Dicer expression may be related to metastatic spread of tumours.

Conclusions:

Assessment of Dicer expression may facilitate prediction of distant metastases for patient suffering from breast cancer.

Note: This abstract was not presented at the AACR 101st Annual Meeting 2010 because the presenter was unable to attend.

Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1442.

Membres

ALAIN PUISIEUX

Professeur - Médecin UVSQ