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- ARF6–JIP3/4 regulate endosomal tubules for MT1-MMP exocytosis in cancer invasion
ARF6–JIP3/4 regulate endosomal tubules for MT1-MMP exocytosis in cancer invasion
Auteurs
Valentina Marchesin, Antonio Castro-Castro, Catalina Lodillinsky, Alessia Castagnino, Joanna Cyrta, Hélène Bonsang-Kitzis, Laetitia Fuhrmann, Marie Irondelle, Elvira Infante, Guillaume Montagnac, Fabien Reyal, Anne Vincent-Salomon, Philippe Chavrier
Résumé
Invasion of cancer cells into collagen-rich extracellular matrix requires membrane-tethered membrane type 1–matrix metalloproteinase (MT1-MMP) as the key protease for collagen breakdown. Understanding how MT1-MMP is delivered to the surface of tumor cells is essential for cancer cell biology. In this study, we identify ARF6 together with c-Jun NH2-terminal kinase–interacting protein 3 and 4 (JIP3 and JIP4) effectors as critical regulators of this process. Silencing ARF6 or JIP3/JIP4 in breast tumor cells results in MT1-MMP endosome mispositioning and reduces MT1-MMP exocytosis and tumor cell invasion. JIPs are recruited by Wiskott-Aldrich syndrome protein and scar homologue (WASH) on MT1-MMP endosomes on which they recruit dynein–dynactin and kinesin-1. The interaction of plasma membrane ARF6 with endosomal JIPs coordinates dynactin–dynein and kinesin-1 activity in a tug-of-war mechanism, leading to MT1-MMP endosome tubulation and exocytosis. In addition, we find that ARF6, MT1-MMP, and kinesin-1 are up-regulated in high-grade triple-negative breast cancers. These data identify a critical ARF6–JIP–MT1-MMP–dynein–dynactin–kinesin-1 axis promoting an invasive phenotype of breast cancer cells.
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