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- Dynamics of CRISPR-Cas9 genome interrogation in living cells
Dynamics of CRISPR-Cas9 genome interrogation in living cells
Auteurs
Spencer C. Knight, Liangqi Xie, Wulan Deng, Benjamin Guglielmi, Lea B. Witkowsky, Lana Bosanac, Elisa T. Zhang, Mohamed El Beheiry, Jean-Baptiste Masson, Maxime Dahan, Zhe Liu, Jennifer A. Doudna, Robert Tjian
Résumé
Genome editing with a Cas9 scalpel
The Cas9 nuclease forms the heart of the CRISPR-Cas genome editing system. Cas9 binds small guide RNAs that direct it to its target sites, where the nuclease either cleaves or binds to genomic DNA. Knight