Apical size and deltaA expression predict adult neural stem cell decisions along lineage progression

Nom de la revue
Science Advances
Laure Mancini, Boris Guirao, Sara Ortica, Miriam Labusch, Felix Cheysson, Valentin Bonnet, Minh Son Phan, Sébastien Herbert, Pierre Mahou, Emilie Menant, Sébastien Bedu, Jean-Yves Tinevez, Charles Baroud, Emmanuel Beaurepaire, Yohanns Bellaiche, Laure Bally-Cuif, Nicolas Dray
Abstract

The maintenance of neural stem cells (NSCs) in the adult brain depends on their activation frequency and division mode. Using long-term intravital imaging of NSCs in the zebrafish adult telencephalon, we reveal that apical surface area and expression of the Notch ligand DeltaA predict these NSC decisions.
deltaA
-negative NSCs constitute a bona fide self-renewing NSC pool and systematically engage in asymmetric divisions generating a self-renewing

deltaA
neg

daughter, which regains the size and behavior of its mother, and a neurogenic

deltaA
pos

daughter, eventually engaged in neuronal production following further quiescence-division phases. Pharmacological and genetic manipulations of Notch, DeltaA, and apical size further show that the prediction of activation frequency by apical size and the asymmetric divisions of

deltaA
neg

NSCs are functionally independent of Notch. These results provide dynamic qualitative and quantitative readouts of NSC lineage progression in vivo and support a hierarchical organization of NSCs in differently fated subpopulations.