• Accueil >
  • Publications >
  • A cell fate decision map reveals abundant direct neurogenesis bypassing intermediate progenitors in the human developing neocortex

A cell fate decision map reveals abundant direct neurogenesis bypassing intermediate progenitors in the human developing neocortex

1 mai 2024Nature Cell Biology

DOI : 10.1038/s41556-024-01393-z

Auteurs

Laure Coquand, Clarisse Brunet Avalos, Anne-Sophie Macé, Sarah Farcy, Amandine Di Cicco, Marusa Lampic, Ryszard Wimmer, Betina Bessières, Tania Attie-Bitach, Vincent Fraisier, Pierre Sens, Fabien Guimiot, Jean-Baptiste Brault, Alexandre D. Baffet

Résumé

Abstract

The human neocortex has undergone strong evolutionary expansion, largely due to an increased progenitor population, the basal radial glial cells. These cells are responsible for the production of a diversity of cell types, but the successive cell fate decisions taken by individual progenitors remain unknown. Here we developed a semi-automated live/fixed correlative imaging method to map basal radial glial cell division modes in early fetal tissue and cerebral organoids. Through the live analysis of hundreds of dividing progenitors, we show that basal radial glial cells undergo abundant symmetric amplifying divisions, and frequent self-consuming direct neurogenic divisions, bypassing intermediate progenitors. These direct neurogenic divisions are more abundant in the upper part of the subventricular zone. We furthermore demonstrate asymmetric Notch activation in the self-renewing daughter cells, independently of basal fibre inheritance. Our results reveal a remarkable conservation of fate decisions in cerebral organoids, supporting their value as models of early human neurogenesis.

Membres

VINCENT FRAISIER

Ingénieur de recherche CNRS

AMANDINE DI CICCO

Ingénieur d'études

JEAN-BAPTISTE BRAULT

Maître de conférences Sorbonne Université

ALEXANDRE BAFFET

Chargé de recherche Inserm