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- Transient cell stiffening triggered by magnetic nanoparticle exposure
Transient cell stiffening triggered by magnetic nanoparticle exposure
Auteurs
Jose E. Perez, Florian Fage, David Pereira, Ali Abou-Hassan, Sophie Asnacios, Atef Asnacios, Claire Wilhelm
Résumé
Abstract
Background
The interactions between nanoparticles and the biological environment have long been studied, with toxicological assays being the most common experimental route. In parallel, recent growing evidence has brought into light the important role that cell mechanics play in numerous cell biological processes. However, despite the prevalence of nanotechnology applications in biology, and in particular the increased use of magnetic nanoparticles for cell therapy and imaging, the impact of nanoparticles on the cells’ mechanical properties remains poorly understood.
Results
Here, we used a parallel plate rheometer to measure the impact of magnetic nanoparticles on the viscoelastic modulus
Conclusions
Overall, this work evidences that magnetic nanoparticle internalization, coupled to cytoskeleton remodeling, contributes to a change in the cell mechanical properties within minutes of their initial contact, leading to an increase in cell rigidity. This effect appears to be transient, reduced after hours and disappearing 24Â h after the internalization has taken place.
Membres
