BAP1 complex promotes transcription by opposing PRC1-mediated H2A ubiquitylation

Nom de la revue
Nature Communications
Antoine Campagne, Ming-Kang Lee, Dina Zielinski, Audrey Michaud, Stéphanie Le Corre, Florent Dingli, Hong Chen, Lara Z. Shahidian, Ivaylo Vassilev, Nicolas Servant, Damarys Loew, Eric Pasmant, Sophie Postel-Vinay, Michel Wassef, Raphaël Margueron

AbstractIn Drosophila, a complex consisting of Calypso and ASX catalyzes H2A deubiquitination and has been reported to act as part of the Polycomb machinery in transcriptional silencing. The mammalian homologs of these proteins (BAP1 and ASXL1/2/3, respectively), are frequently mutated in various cancer types, yet their precise functions remain unclear. Using an integrative approach based on isogenic cell lines generated with CRISPR/Cas9, we uncover an unanticipated role for BAP1 in gene activation. This function requires the assembly of an enzymatically active BAP1-associated core complex ( containing one of the redundant ASXL proteins. We investigate the mechanism underlying transcriptional regulation and show that it does not participate in Polycomb-mediated silencing. Instead, our results establish that the function of is to safeguard transcriptionally active genes against silencing by the Polycomb Repressive Complex 1.