Spatial Omic

The tissue is made up of heterogeneous cell types, each with specific functions and functional states and whose spatial organization can have a strong impact on their respective roles.

CYTPIC thus has a high-resolution, whole-slide spectral, multimodal imaging instrument. A combination of simultaneous and quantitative immunofluorescence (up to 17 fluorescences at the same time) coupled with bright field imaging (Ex: H&E) allows spatial biology studies and robust quantification of biomarkers. Beyond the identification of cell subpopulations, this approach provides information on the spatial location of the subpopulations identified within the tissue, their proximity to each other and the interactions with the extracellular matrix, blood vessels and tissues. other tissue components.
Data analysis is carried out jointly with the Bioinformatics platform of the Institut Curie.

To enhance tissue proteomic analysis, CYTPIC also features a single-cell spatial imaging instrument for transcriptomic analysis. This seamlessly integrates the workflow of transcript detection, high-resolution imaging, decoding, and onboard data analysis. Using multimodal cell segmentation, this instrument can analyze 472 mm² of tissue per run, targeting up to 480 genes in less than 3 days or up to 5,000 genes in less than 6 days.

Paris

Rarecyte Orion up to 20 colors. 7 lasers : 405nm, 445nm, 470nm, 520nm, 555nm, 640nm and 730nm. Magnification : 4x, 10x,20x and 40x.

10x Xenium up to 2 slides per cycle. 472 mm² of tissue per cycle, up to 480 genes in < 3 days or up to 5,000 genes in < 6 days.

*CYTPIC's activities are open to interns and externs, autonomously and as a service, please contact cytometrie@curie.fr for more information.